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多种灭活益生菌静脉注射对小鼠免疫功能六合图库研究
发布时间:2019-03-20



兽医硕士香港六合资料范文第二篇:多种灭活益生菌静脉注射对小鼠免疫功能六合图库研究

  
  本篇文章目录导航:

  【题目】多种灭活益生菌静脉注射对小鼠免疫功能六合图库研究
  【第一章】对灭活菌研究的前言
  【第二章】对小鼠灭活益生菌的实验
  【第三章】灭活益生菌对小鼠免疫功能的实验结果
  【第四章】益生菌静脉注射的作用讨论
  【第五章-参考文献】静脉注射灭活益生菌对小鼠免疫功能六合图库的结论

中文摘要

  
  益生菌是一类微生物活菌制剂,能够通过调节动物肠道微生态平衡,促进宿主动物的健康和生长。益生菌还具有增强机体免疫功能、改善肝脏疾病和抑制肿瘤生长等作用。目前多种益生菌制剂被广泛应用于生物工程、农业、食品以及生命健康领域。
  
  本研究主要对灭活酵母菌(酿酒酵母和德尔布有孢圆酵母)、灭活芽孢菌(枯草芽孢杆菌和地衣芽孢杆菌)、灭活丁酸梭菌、灭活乳酸菌(乳酸乳球菌乳酸亚种、植物乳杆菌植物亚种,长双歧杆菌、屎肠球菌和短乳杆菌)及灭活乳酸菌混合注射剂进行了研究,探究了各种灭活益生菌静脉注射对小鼠免疫功能及抗菌进行的六合图库。主要研究结果如下:
  
  灭活酵母菌静脉注射对小鼠免疫功能和抗菌作用的六合图库。将灭活酿酒酵母注射剂和灭活德尔布有孢圆酵母注射剂分别尾静脉注射正常和沙门菌感染小鼠,检测小鼠的免疫功能和抗感染作用。结果发现,灭活酿酒酵母和灭活德尔布有孢圆酵母对小鼠的脾指数和胸腺指数无显着六合图库,但二者分别显着和极显着地提高了正常小鼠的碳廓清指数K值,即提高了正常小鼠的单核-巨噬细胞吞噬功能。灭活酿酒酵母注射剂极显着提高了小鼠血清中TNF-α和IFN-γ含量,灭活德尔布有孢圆酵母注射剂组对TNF-α无显着性六合图库,并显着降低血清中IFN-γ的含量。抗菌实验发现,灭活酿酒酵母注射剂和灭活德尔布有孢圆酵母注射剂均极显着地降低了小鼠死亡数。以上说明灭活酿酒酵母注射剂和灭活德尔布有孢圆酵母注射剂静脉给药对小鼠免疫功能具有调节作用,对沙门氏菌感染小鼠具有一定的保护作用。
  
  灭活芽孢菌静脉注射对小鼠免疫功能和抗菌作用的六合图库。将灭活枯草芽孢杆菌注射剂和灭活地衣芽孢杆菌注射剂分别尾静脉注射正常和沙门菌感染小鼠,检测小鼠的免疫功能和抗感染作用。灭活枯草芽孢杆菌注射剂能够极显着提高小鼠脾指数,而对胸腺指数无显着六合图库;灭活地衣芽孢杆菌对小鼠脾指数和胸腺指数均无显着六合图库。灭活枯草芽孢杆菌注射剂和地衣芽孢杆菌注射剂静脉注射给药,对小鼠的单核-巨噬细胞系统的吞噬功能无显着六合图库。灭活地衣芽孢杆菌注射剂组与正常对照组相比较,能够分别使血清中TNF-α和IFN-γ含量显着和极显着地提高,但灭活枯草芽孢杆菌注射剂对小鼠血清中TNF-α和IFN-γ含量无显着六合图库。抗菌实验发现,灭活枯草芽孢杆菌注射剂和灭活地衣芽孢杆菌注射剂均极显着地降低小鼠的死亡数。以上说明二者静脉给药对小鼠的免疫功能具有调节作用,并对沙门氏菌感染小鼠具有一定的保护作用。
  
  灭活丁酸梭菌静脉注射对小鼠免疫功能和抗菌作用的六合图库。将灭活丁酸梭菌注射剂尾静脉注射正常和沙门菌感染小鼠,检测小鼠的免疫功能和抗感染作用。灭活丁酸梭菌注射剂对小鼠的免疫器官指数无显着六合图库;对正常小鼠的碳廓清指数K无显着六合图库,说明其对小鼠的单核-巨噬细胞系统的吞噬功能无显着六合图库,但灭活丁酸梭菌注射剂能够显着地提高小鼠血清中TNF-α含量,而对IFN-γ含量无显着六合图库。抗菌实验发现,灭活丁酸梭菌极显着地降低了小鼠的死亡数,说明其静脉给药对沙门氏菌感染小鼠具有一定的保护作用。
  
  灭活乳酸菌静脉注射剂对小鼠免疫功能和抗菌作用的六合图库。将灭活乳酸乳球菌乳酸亚种注射剂、灭活长双歧杆菌注射剂、灭活短乳杆菌注射剂、灭活植物乳杆菌植物亚种注射剂、灭活屎肠球菌注射剂和2种灭活乳酸菌混合注射剂分别尾静脉注射正常和沙门菌感染小鼠,检测小鼠的免疫功能和抗感染作用。结果发现,灭活乳酸乳球菌乳酸亚种注射剂、灭活长双歧杆菌注射剂和灭活短乳杆菌注射剂三者可以显着地提高小鼠的碳廓清指数K值,而灭活植物乳杆菌植物亚种注射剂、灭活屎肠球菌注射剂和2种灭活乳酸菌混合注射剂均能够极显着提高小鼠的碳廓清指数K值。因此,静脉注射5种不同灭活乳酸菌及混合注射剂均可以提高小鼠的非特异性免疫功能。抗菌实验表明,灭活乳酸乳球菌乳酸亚种注射剂、灭活植物乳杆菌植物亚种注射剂、灭活长双歧杆菌注射剂、灭活屎肠球菌注射剂和2种灭活乳酸菌注射剂均能够极显着降低小鼠的死亡率,而灭活短乳杆菌注射剂能够显着地降低小鼠的死亡率,该实验说明乳酸菌静脉注射剂对小鼠感染肠炎沙门氏菌有良好的保护作用。
  
  综上所述,灭活酵母菌(酿酒酵母和德尔布有孢圆酵母)、灭活芽孢菌(枯草芽孢杆菌和地衣芽孢杆菌)、灭活丁酸梭菌、灭活乳酸菌(乳酸乳球菌乳酸亚种、植物乳杆菌植物亚种、长双歧杆菌、屎肠球菌和短乳杆菌)及灭活乳酸菌混合注射剂均能够通过不同途径增强机体的免疫功能,且对抗小鼠感染沙门菌作用较好。因此,本研究为新型益生菌免疫增强剂的研发提供了理论依据。
  
  关键词:益生菌; 免疫功能;肠炎沙门氏菌;抗菌作用

小鼠

Abstract

  
  Probiotics is a kind of microbial living bacteria preparation,Can the intestinalmicroecological balance by regulating animal,Promote the health of the host animal andgrow.Probiotics also can strengthen the body's immune function, improve liver disease andtumor growth inhibition.At present many kinds of probiotic preparations are widely used inbiological engineering, agriculture, food and health life. In this study, inactivated Yeasts(Saccharomyces cerevisiae and D. delbrunis), inactivated Sporeforms (Bacillus subtilis andBacillus licheniformis), inactivated Clostridium Butyricum, inactivated Lactobacillus(Lactococcus lactis subspecies, Lactobacillus plantarum subspecies, Bifidobacterium longum,Enterococcus faecium and Lactobacillus brevis) and its mixture were injected into the tailvein of normal mice and mice infected with Salmonella to explore the effects of variousinactivated probiotics on immune function and antibacterial activity of mice, * * indicatorswere determined by * * method. The results demonstrated as bellow:
  
  (1) Effects of inactivated Yeast intravenous injection on immune function andantibacterial action in mice. The results of immunological function tests showed that, bothinactivated Saccharomyces cerevisiae and inactivated Torulaspora delbrueckii intravenousfluid had no effect on the spleen and thymus, while K-value increased. The serum TNF-α andIFN-γ were increased by inactivated Saccharomyces cerevisiae. These results demonstratedthat these two intravenous fluid could enhance the immune function, and the inactivatedSaccharomyces cerevisiae was better. Antibacterial test results showed that, both inactivatedSaccharomyces cerevisiae and inactivated Torulaspora delbrueckii intravenous fluidprotected against the Salmonella infection and the death rate was reduced.
  
  (2) Effects of inactivated Sporeforms intravenous injection on immune function andantibacterial action in mice. The results of immunological function tests showed that, thespleen weight was increased by inactivated Bacillus subtilis. The serum TNF-α and IFN-γwere increased by inactivated Bacillus licheniformis. These results demonstrated that thesetwo intravenous fluid could enhance the immune function by different pathway. Antibacterialtest results showed that, both inactivated Bacillus subtilis and Bacillus licheniformisintravenous fluid protected against the Salmonella infection and the death rate was reduced.
  
  (3) Effects of inactivated Clostridium Butyricum intravenous injection on immunefunction and antibacterial action in mice. The results of immunological function tests showedthat, no effects was determined on immune organs and K-value by Clostridium Butyricumintravenous fluid, while serum TNF-α was increased. These results demonstrated thatClostridium Butyricum intravenous fluid could enhance the immune funtion. Antibacterial testresults showed that, both inactivated Clostridium Butyricum intravenous fluid portectedagainst the Salmonella infection and the death rate was reduced.
  
  (4) Effects of inactivated Lactobacillus and its mixture intravenous injection onimmune function and antibacterial action in mice. The results of immunological function testsshowed that, the K-value was increased by these 6 different types of probiotics, andLactobacillus plantarum subsp. Plantarum, Enterococcus faecium and the mixed probioticswere much better. Antibacterial test results showed that, all the inactivated intravenous fluidcould protect against the Salmonella infection and the death rate was reduced.
  
  To sum up, inactivated Yeasts, inactivated Sporeforms, inactivated ClostridiumButyricum, inactivated Lactobacillus and its mixture could enhance the immune function ofmice through different ways, and protect mice against Salmonella. This study suppliedessential data for development of immunopotentiator and antimicrobial agents.
  
  Keywords: Probiotics; Immune; Salmonella infection; Antibacterial action

目 录

  中文摘要
  Abstract

  1 前言
  1.1 益生菌研究进展
  1.1.1 酵母菌研究概况
  1.1.2 益生芽孢菌研究概况
  1.1.3 丁酸梭菌研究概况
  1.1.4 乳酸菌研究概况
  1.2 免疫调节剂的研究进展
  1.3 本课题的研究意义

  2 实验部分
  2.1 实验材料
  2.1.1 实验动物
  2.1.2 实验菌种
  2.1.3 化学试剂
  2.1.4 仪器设备

  2.2 实验方法
  2.2.1 多种灭活益生菌注射液的制备
  2.2.1.1 灭活酵母菌注射剂的制备方法
  2.2.1.2 灭活芽孢菌注射剂的制备方法
  2.2.1.3 灭活丁酸梭菌注射剂的制备方法
  2.2.1.4 灭活乳酸菌注射剂的制备方法

  2.2.2 多种灭活益生菌静脉注射对小鼠免疫功能的六合图库研究
  2.2.2.1 实验分组及给药
  2.2.2.2 采用器官指数评价药物对免疫器官的六合图库
  2.2.2.3 采用碳粒廓清法检测小鼠单核-巨噬细胞系统吞噬功能
  2.2.2.4 采用放射免疫法检测血清中 TNF-α和 IFN-γ含量
  2.2.3 多种益生菌静脉注射对小鼠感染沙门氏菌的作用研究
  2.2.4 数据处理

  3 实验结果
  3.1 灭活酵母菌静脉注射剂对小鼠的作用研究
  3.1.1 灭活酵母菌静脉注射对小鼠免疫功能的作用结果
  3.1.1.1 灭活酵母菌静脉注射对小鼠免疫器官指数的作用结果
  3.1.1.2 灭活酵母菌静脉注射对小鼠单核-巨噬细胞系统的作用结果
  3.1.1.3 灭活酵母菌静脉注射对小鼠血清中 TNF-α和 IFN-γ含量的六合图库结果
  3.1.2 灭活酵母菌静脉注射对小鼠抗菌作用的结果

  3.2 灭活芽孢菌静脉注射剂对小鼠的作用研究
  3.2.1 灭活芽孢菌静脉注射对小鼠免疫功能的作用结果
  3.2.1.1 灭活芽孢菌静脉注射对小鼠免疫器官指数的作用结果
  3.2.1.2 灭活芽孢菌静脉注射对小鼠单核-巨噬细胞系统的作用结果
  3.2.1.3 灭活芽孢菌静脉注射对小鼠血清中 TNF-α和 IFN-γ含量的六合图库结果
  3.2.2 灭活芽孢菌静脉注射对小鼠抗菌作用的结果

  3.3 灭活丁酸梭菌静脉注射剂对小鼠的作用研究
  3.3.1 灭活丁酸梭菌静脉注射对小鼠免疫功能的作用结果
  3.3.1.1 灭活丁酸梭菌静脉注射对小鼠免疫器官指数的作用结果
  3.3.1.2 灭活丁酸梭菌静脉注射对小鼠单核-巨噬细胞系统的作用结果
  3.3.1.3 灭活丁酸梭菌静脉注射对小鼠血清中 TNF-α和 IFN-γ含量的六合图库结果
  3.3.2 灭活丁酸梭菌静脉注射对小鼠抗菌作用的结果

  3.4 灭活乳酸菌静脉注射剂对小鼠的作用研究
  3.4.1 灭活乳酸菌静脉注射对小鼠单核-巨噬细胞系统的六合图库结果
  3.4.2 灭活乳酸菌静脉注射对小鼠抗菌作用的结果

  4 讨论
  4.1 灭活酵母菌静脉注射剂对小鼠的作用
  4.2 灭活芽孢菌静脉注射剂对小鼠的作用
  4.3 灭活丁酸梭菌静脉注射剂对小鼠的作用
  4.4 灭活乳酸菌静脉注射剂对小鼠的作用

  5 结论
  6 创新点
  参考文献

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